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Product Information :

Product Name:  
High Throughput Colorimetric GTPase Assays (inc. PiColorLock?)
Product Category:   เครื่องมือวิทยาศาสตร์ / สารเคมีและวัสดุภัณฑ์สำหรับห้องปฏิบัติการ
Product Sub-Category:   ATPase & GTPase assay kits
Brand:   Expedeon 
Product Type :   สินค้า, ผลิตภัณฑ์ 
Short Description :   High Throughput Colorimetric GTPase Assays (inc. PiColorLock?)
     
 

High Throughput Colorimetric GTPase Assays (inc. PiColorLock™)

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock™ reagent and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock™, a non-radioactive, superior phosphate detection reagent.

Key features of PiColorLock™ include:

  • Colorimetric Assay – competitor assays are radioactive
  • Special additive ensures low backgrounds with acid-labile substrates
  • Unparalleled stability of phosphate-dye complexes
  • Reagent is compatible with almost any assay buffer
  • No inhibition of color development by high concentrations of protein
  • Stable reagent formulation – long shelf life

Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color- see below).

Kit Components:

Components in the 2-plate GTP assay kit (5-plate kit amounts in brackets)

Store at 4°C:

  • 1 x 10ml of PiColorLock™* (1 x 25ml)
  • 1 x 0.25ml of Accelerator (1 x 0.5ml)
  • 1 x 5ml of Stabiliser (1 x 10ml)
  • 1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)
  • 1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)
  • 1 x 5ml of 0.1mM Pi standard (1 x 10ml)

*Take care, the solution is very acidic

Store at -20 or -70oC:

  • 4 vials of lyophilized GTP (10 vials)
  • Store at room temperature
  • 2 x 96-well plates (5 plates)

The data below is for the PiColorLock™ reagent which is included in GTPase assay kits.

Figure 1. Our PiColorLock has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.

 

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