Universal Lateral Flow Assay Kit

For the Fast & Easy Development of Customized Lateral Flow Assays

The Innova Biosciences Universal Lateral Flow Assay (LFA) Kit is designed to enable the easy development of customized sandwich lateral flow assays. The great advantage of this kit is its adaptability to any pair of capture and detection antibodies, which allows the detection of any type of analyte* without the need spray down capture antibodies on the test strip, a labor-intensive process that consumes large amounts of expensive antibody reagent.

• Fully customizable – adaptable to any pair of capture and detection antibodies and can detect any type of analyte*
• Quick & easy to use – both conjugations can be set up in only 30 seconds
• No false negative results – assay is compatible with biological samples
• No specialized or costly equipment
• Can be qualitatively and quantitatively analyzed using the supplied scoring card or a LFA reader respectively

* The antigen must contain at least two antigenic sites for the binding of the capture and detection antibodies

The kit combines Innova’s easy to use Lightning-Link® antibody labeling and InnovaCoat® GOLD nanoparticle conjugation technologies with an immunochromatographic test performed on Universal-LFA strips. The capture antibody is conjugated to Lightning-Link® Ulfa-Tag, while the detection antibody is conjugated to 40nm InnovaCoat® GOLD, both of which require only 30 seconds to set up.

The capture and detection antibodies are diluted and incubated with the analyte and 40nm InnovaCoat® GOLD-Biotin and then run on Universal LFA strips. Universal LFA strips consist of a nitrocellulose membrane containing a ‘Test line’ (T-line) of immobilized anti- Ulfa-Tag antibody, that binds the Ulfa-Tag-conjugated capture antibody which further binds the analyte in complex with the InnovaCoat GOLD®-detection antibody. A red T-line appears when the analyte is present and the line intensity varies depending on the analyte concentration (see Figure 1). Universal LFA strips also contain a ‘Control-line’ (C-line) striped with streptavidin, which confirms that the test is valid, and an absorbent pad to promote and control the flow of sample through the membrane.

Kit components

3x 100ug Lightning-Link®  Ulfa-Tag conjugation kit

• Targets amine groups
• Conjugate up to 3 different capture antibodies
• Not suitable for nucleic acid or small molecule conjugation
• Ulfa-Tag is used in many molecular biology applications

3x MINI reaction 40nm InnovaCoat® GOLD

• Conjugate up to 3 different detection antibodies
• Conjugate is ready to use in just 20 minutes
• Forms highly stable conjugates
• Proprietary surface coating prevents metal-protein interactions

40nm InnovaCoat GOLD®-Biotin

100 Universal-LFA strips

Buffer

2 x 96-well low binding well plates

Scoring card

Figure 1. Example of sandwich LFA assay for the detection of CRP in serum using the Universal LFA kit: Lighting-Link® Ulfa-Tag and 40nm IC-GOLD are used to conjugate the capture and detection antibody respectively. Results were analysed using Qiagen ESEQuant reader.